Functional evolution of C4 pyruvate, orthophosphate dikinase
نویسندگان
چکیده
منابع مشابه
Functional evolution of C(4) pyruvate, orthophosphate dikinase.
Pyruvate,orthophosphate dikinase (PPDK) plays a controlling role in the PEP-regeneration phase of the C(4) photosynthetic pathway. Earlier studies have fully documented its biochemical properties and its post-translational regulation by the PPDK regulatory protein (PDRP). However, the question of its evolution into the C(4) pathway has, until recently, received little attention. One assumption ...
متن کاملPyruvate orthophosphate dikinase from the immature grains of cereal grasses.
Pyruvate orthophosphate dikinase has been identified in the green grains of eight cereal grasses, most of which are classified as C(3) plants. The wheat (Triticum aestivum L. cv. Lerma Rojo) grain enzyme was further investigated: activity was low in very young grains, increased to a maximum at about 25 days after anthesis, then returned to a low level as the grain matured. It appeared to be loc...
متن کاملPyruvate orthophosphate dikinase gene expression in developing wheat seeds.
The amount of pyruvate orthophosphate dikinase (PPDK) (EC 2.7.9.1) protein in wheat (Triticum aestivum L. var Cheyenne) grains was determined at different stages of development by the protein blot method. The variation in PPDK protein with time in developing wheat grains was similar to that of the enzyme's activity reported by Meyer et al. (1982 Plant Physiol 69: 7-10). The variation in levels ...
متن کاملThe promoter for the maize C4 pyruvate, orthophosphate dikinase gene directs cell- and tissue-specific transcription in transgenic maize plants.
The pyruvate,orthophosphate dikinase (PPDK) gene coding the chloroplast enzyme involved in C4 photosynthesis has a dual promoter system. The first promoter is responsible for the transcription of a larger transcript and its product is targeted to the chloroplast (hence, it is designated as C4Pdk promoter) while the second promoter is responsible for the transcription of a smaller transcript and...
متن کاملPyruvate orthophosphate dikinase of c(3) seeds and leaves as compared to the enzyme from maize.
Pyruvate orthophosphate dikinase (PPDK) was found in various immature seeds of C(3) plants (wheat, pea, green bean, plum, and castor bean), in some C(3) leaves (tobacco, spinach, sunflower, and wheat), and in C(4) (maize) kernels. The enzyme in the C(3) plants cross-reacts with rabbit antiserum against maize PPDK. Based on protein blot analysis, the apparent subunit size of PPDK from wheat seed...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2011
ISSN: 1460-2431,0022-0957
DOI: 10.1093/jxb/err058